This manne can litle skyl ... to saue himself harmlesse from the perilous accidentes of this world, keping ye wulf from the doore (as they cal it). ["The Institution of a Gentleman," 1555]
Wolves as a symbol of lust are ancient, e.g. Roman slang lupa "whore," literally "she-wolf" (preserved in Spanish loba, Italian lupa, French louve). The equation of "wolf" and "prostitute, sexually voracious female" persisted into 12c., but by Elizabethan times wolves had become primarily symbolic of male lust. The specific use of wolf for "sexually aggressive male" first recorded 1847; wolf-whistle first attested 1952. The image of a wolf in sheep's skin is attested from c.1400. See here for a discussion of "wolf" in Indo-European history.